| ≥1 piece |
Avidin is a strongly basic glycoprotein synthesized in the hen oviduct and deposited in the albumen fraction of eggs at a ratio of 0.05% of the total protein content. It binds the water-soluble vitamin H (d-biotin) very tightly and specifically with a binding log constant of 15. Avidin is a tetrameric protein, composed of subunits of identical amino acid composition and sequence. Each monomer is glycosylated on Asn-17. Avidin from Belovo is a white free flowing lyophilised powder, free of any visible impurities.
The high affinity constant of Avidin for Biotin has been widely used in molecular biology: affinity chromatography, molecular recognition and labelling, Enzyme Linked ImmunoSorbent Assay (ELISA), histochemistry and cytochemistry. An excellent review in the field has been written by Wilchek, M. and Bayer, E.A., eds. (1990), 'Avidin-Biotin Technology', Methods in Enzymology, Vol.184, Academic Press.