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As a necessary amino acids to human and animal life, l-tryptophan plays an important role in metabolism, thus it is referred as the second necessary amino acid. It is widely used in medicine, food, feed and so on. In living organisms, L-tryptophan can be compounded to 5-hydroxy tryptamine and other hormones as well as pigments, alkaloids, coenzymes, plant hormones and other physiologically active substances, which can prevent and treat pellagra as well as to eliminate stress, improve the efficacy of sleep effects, Tryptophan metabolic disorders will cause diabetes and neurotic disorders, therefore in medicine it can be used in injection solution of amino acid and amino acid compound, In addition, some plants lack of L-tryptophan, so it is used as food and feed additives to improve the utilization of plant proteins. It is the third-largest feed amino acids after the methionine and lysine