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Nisin is a polypeptide produced by some strains of Streptococcus Lactic (later termed Lactococcus lactis subspecies lactis ) strain, which contains 34 amino acids. It is a natural anti-microbial agent and used as a natural food preservative with high efficiency, nontoxic, no harm to humans and be free from side effects and is deemed to be a GRAS (Generally Recognised as Safe) substance. Nisin is soluble in aqueous environments and most stable in acid conditions.