5'-GUANYLIC ACID,China UC price supplier

SYNONYMS

Guanylic acid, GMP, INS No. 626

DEFINITION

Chemical names

Guanosine-5'-monophosphoric acid

C.A.S. number

85-32-5

Chemical formula

C ₁₀ H ₁₄ N ₅ O ₈ P

Structural formula

Formula weight

363.22

Assay

Not less than 97.0% and not more than 102.0% of on the dried basis

DESCRIPTION

Odourless, colourless or white crystals, or a white crystalline powder

FUNCTIONAL USES

Flavour enhancer

CHARACTERISTICS

IDENTIFICATION

Solubility  (Vol. 4)

Slightly soluble in water; practically insoluble in ethanol

Spectrophotometry

A  1 in 50,000 solution of the sample in 0.01 N hydrochloric acid exhibits an absorbance maximum at 256¡À2nm. The ratio A250/A260 is between 0.95 and 1.03, and the ratio A280/260 is between 0.63 and 0.71.

Test for ribose (Vol. 4)

Passes test

Test for organic phosphate  (Vol. 4)

Passes test

Test 5 ml of a  1 in 400 soln

PURITY

Loss on drying  (Vol. 4)

Not more than 1.5% (120 ^o , 4 h)

pH  (Vol. 4)

1.5 - 2.5 ( 1 in 400 soln)

Related foreign substances (Vol. 4)

Chromatographically not detectable

Test 2 µl of a  1 in 400 soln

Lead  (Vol. 4)

Not more than 1 mg/kg

Determine using an atomic absorption technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4, ¡°Instrumental Methods.¡±

METHOD OF ASSAY

Weigh accurately about  0.5 g of the sample, dissolve in and make to 1,000 ml with 0.01 N hydrochloric acid. Take 10 ml of this solution and dilute with 0.01 N hydrochloric acid to 250 ml. Determine the absorbance A of the solution in a 1-cm cell at the wave length of 260 nm using 0.01 N hydrochloric acid as the reference.

Calculate the content of C ₁₀ H ₁₄ N ₅ O ₈ P, in % in the sample by the formula: