Nisin
Nisin is a polypeptide produced by Lactococcus lactis and can be digested into amino acids by a-chymotrypsinin in human intestines and stomach. The use of Nisin will not affect the normal bacterial community, nor produce the drug resistance or chiasmatic resistance as it happened when the antibiotics used. Nisin is a nonpoisonous and high efficacious Food Preservative/ anti-microbial agent. It possesses anti-microbial activity against a wide range of Gram-positive bacteria, particularly those that produce spores, which are the major food spoilage organisms, such as Clostridium botulinum, Staphylococcus aureus, Streptococcus hemolyticus, Listeria monocytogenes, Bacillus stearothermophilus, Bacillus subtilis etc. The use of Nisin, as a food preservative, can greatly decrease the required temperature and shorten the time in food heat processing, which could not only improve the nutritional value, appearance, flavor and texture of foods, significantly prolong the shelf-life, but also save energy greatly and lower the product cost. Now, Nisin has been used in a wide range of processed foods, e.g. cured meat, dairy products, plant protein foods, canned foods, and heat-treated/air-tightly packed foods etc, it can also be used in the area of cosmetics, medicines and health products.
Technical Data of the Product
Product Name: WeiRi-Brand Nisin
Molecular Formula: C143H228O37N42S7
Molecular Weight: 3348
Product code: China ： 17.019
INS ： 234
CAS ： 1414-45-5
Specification of Product
Program Specification
Appearance Off-white powder
Content (IU/mg) ≥ 1000
Moisture(%) ≤ 3
Heavy Metals (Pb) (mg/kg) ≤ 0.001 ≤ 10
As(%) ≤ 0.0001
NaCl (%) ≥ 50
Count of Colony (cuf/g) ≤ 10
E. coli. (MPN/100g) ≤ 30
Salmonellae spp. Absent in 25g
Stability
Nisin is most stable under acid conditions. It can keep the activity after the treatment at 121 ℃ for 30mins at pH2; 47% of the activity remained after the treatment in 110 ℃ for 30mins in nonfat milk in pH6.5; But the activity lost rapidly in 30 minutes at 63 ℃ in pH 11.
Solubility
Nisin can be dissolved in aqueous solution, but is insoluble in non-polar solvents. The solubility decreases with the increase of pH, and it increases with the rise of temperature.
Anti-microbial Properties
Nisin possesses highly of inhibiting activities to Gram-positive bacteria ， particularly to spore- forming bacteria. It does not influence Gram-negative bacteria, yeasts and moulds generally. But under some circumstances (such as freezing, heating, low pH and adding of EDTA), Nisin can inhibit some Gram-negative bacteria such as Salmonella spp, Shigella spp, Klebsiell spp Escherichia coli etc.
Direction of Usage
About 5% aqueous solution is prepared firstly with cold boiled water or distilled water (best with diluted acid solution), then instantly put it into food and stirs well. Or put it directly into food and stirs well.
Recommended Dosage
The general dosage is 0.05g/kg-0.1g/kg. The detail dosage of Nisin depends on products, material quality, process, shelf life and storage conditions, etc.
Packing and Storage
A. Packing:
WeiRi-brand Nisin is supplied in 500g/bottle, 1000 g/bottle with self-sealed screw-caps, 10 bottles/case 20 bottles/case, or packed at customer′s request.
B. Storage:
WeiRi-brand Nisin is stable for 24 months from the date of manufacture when it is stored in dry conditions, away from direct sunlight and below 10 ℃ .
Natamycin
Natamycin (pimaricin) is a polyene macrolide antifungal agent produced by Streptomyces natalensis, Streptomyces chatanoogen and some other Streptomyces spp. It is the sole antifungal food bio-preservative approved in the world, which could inhibits the growth of moulds and yeasts broadly and high-effectively and is safe to human body. The use of Natamycin does not change the nutritional value, appearance, flavor and texture of foods. Until now, Natamycin has been approved as food preservative in more than 40 countries and applied widely in cheese, meat food, cakes, fruit juice/sauce/salad, salted products and other foods. In China, Natamycin was approved as food preservative in 1996 and could be used in cheese, meat food, Guangdong-flavor-moon cakes, cake surface, fruit juices and easily-mouldy food processing devices et al. The residue of Natamycin is less than 10mg/kg.
Safety and Toxicity
Natamycin is nontoxic to human bodies, and no any carcinogenesis, mutagenesis or hypersensitivity reaction (HSR) was observed after its use. Natamycin could not be obsorbed in mammalian alimentary canal. No normal reistance of moulds and yeasts to natamycin was not observed. ADI 0~0.3mg/kg (FAO/WHO, 1994). Safety to apply on foods (FDA, 172.155,1994 ）
Solubility
The solubility of Natamycin is very low in water, and a little higher in ethanol. Natamycin could dissolve in low concentration.of HCl, Glacial Acetic Acid and dimethylsulfoxide etc., but is insoluble in most organic solvents. The solubility of Natamycin i n water is about 30~100mg/L at room temperature. It will increases when pH ≥ 9 or ≤ 3, which will decrease the antifungal activity.
Net Weight:
250g/bottle,500g/bottle
Net Weight:
250g/bottle,500g/bottle
Gross Weight:
290g/bottle,560g/bottle
Gross Weight:
290g/bottle,560g/bottle
Physical state: powder.
Appearance: white to almost white
Odor: odorless.
Solubility: n/a
Other: n/f
Stability
Many factors can influence the stability of Natamycin:Temperature, pH value, Light, Oxygen,heavy metals.
Packing and Storage
A. Packing:
WeiRi-brand Natamycin is supplied in 250 g/bottle with self-sealed screw-caps, 10 bottles/case 20 bottles/case, or packed at customer′s request.
B. Storage:
WeiRi-brand Natamycin is stable for 24 months from the date of manufacture when it is stored in dry conditions, away from direct sunlight and below 10 ℃ .
ε -Polylysine
ε -Polylysine is a natural substance from Streptomyces albulus metabolism. From 25 ~ 30 lysine residues in polymerization. ， It can inhibit the growth of Gram-positive and Gram-negative bacteria, yeasts, moulds, virus ect. especially inhibit the Gram-negative bacteria which can’t be easily inhibited by other preservatives, such as E. coli, salmonella, and also can inhibit the thermophilics, such as B,stearothermophilus. It is widely used as safe food preservative.
Product Features
ε -Polylysine is highly hygroscopic pale yellow powder with a slightly bitter taste. It is soluble in water, slightly soluble in ethanol, insoluble in organic solvent such as ethyl acetate, ether, ect. as food preservative, ε-Polylysine has following properties.
1) Wide spectrum: ε-Polylysine shows anti-microbial activity with a wide spectrum. it
can inhibit Gram-positive, Gram-negative, Yeasts, Moulds which cause food poisoning and corruption
2) High Safety: After human consumption, ε-Polylysine may degrade L-lysine that is essential amino acids for human, without any toxicity, and it was generally recognized as a nutritional preservative by FDA in 2003.
3) Thermal stability: ε -Polylysine is stable when heated(120 ℃ for 20 min and 80 ℃ for 60 min).it can be sterilized along with the raw material.
4) pH range: ε-Polylysine has little effect on the antibacterial activity against E. coli when pH 3-9. the best pH range is 5-8.this working range is just about other preservative doesn’t work, also most of food pH range.
5) High water solubility: ε-Polylysine is water soluble, it is fit to add to food.
Range of application
ε -Polylysine was generally recognized as a safe food preservative by FDA in October,2003. It is widely used in food preservation. In food application, it is usually applied with alcohol, organic acids, or glyceride, etc.. It can be used in cooked rice, cakes, snacks, noodles, drinks, brewing, meat products, canned food, etc. Also through the Maillard reaction, ε-poly-L-lysine has emulsification and preservation difunctional food additives
Usage
ε -Polylysine is dissolved in cold boiled water or distilled water to 5-10% , then put it into the food according to the recommended dosage and mix fully. If it is used together with other food preservatives, it will can affect better.
Packaging; 0.5kg, 1kg,5kg or packed to customer’s requirements.
Storage; store in well-closed container, away from direct sunlight.