β-glucosidase,China price supplier

company profile

Sunson Industry Group Co., Ltd.

China

Contact: Mr.Albert Sun

Tel: 86-10-58603850

Number of Employees: > 5000

Business Type: Manufacture

product details

Origin:china

beta-Glucosidase

PRODUCT INFORMATION

Introduction

β-glucosidase (also named Cellobiase) is made from Aspergillus niger through cultivation and extraction technique. . It belongs to hydrolytic enzyme with the name of β-D-glucoside hydrolase or gentiobiase. It also contains strong glycoside activity.

Specifications/ PHYSICAL AND CHEMICAL PROPERTIES

Appearance

Colour

Odour

pH

: Liquid with low subsidence

: Brown

: Normal fermentation odour

: Stable 3.5-5.5, optimum 4.0-5.0

Enzymatic Activity

: ≥600 u/mL

Activity Temperature

: Stable at 40-65℃, optimum at 50-60℃

Bulk Density

Calcium ion consistency

: ≤1.25g/mL

: Calcium ion can protect the product

definition of unit

1 unit of β-glucosidase equals to the amount of enzyme which hydrolyzes salicin to get 1µmol reducing sugar （in glucose）at 50℃ and pH4.6 in 1min.

application

1 Production of gentiooligaccharide

Enzymatic production of gentiooligaccharide mainly use specificity of cellobiase of condensation reaction, in high concentration of glucose as raw material, it can compose preferentially gentiooligaccharide of gentiobiose and gentianose etc.

In this way, we can get syrup which contain high and low gentian sugar, in which there are mostly glucose apart from gentiooligaccharide

2 production of cellulase of eficient induction

The gentiobiose,sophorose,sorbose,cellobiose etc which produced by glucose that transformed by glucosidase to glycosylation, is inducer of cellulase synthesis. It can increase 20% fermentation activity of cellulase, improve the brightness of liquid enzyme,improve the comprehensive quality of cellulase.

3 Improve the flavor

Owing to most of precursor is in a bound form of the glycoside and no aromatic odor, BGL can work togerther with other flavor enzyme to work on β-glucoside which can break down combined fragrant material and release free fragrant material, so it can increase the aroma effect and natural flavor of fruit juice etc.

4 Extraction of polydatin

Using beta glucosidase conversion technology, we can hydrolyze resveratrol with high content glucoside in the polydatin into free resveratrol, thus greatly improve the yield of resveratrol.

5 Debittering effect

It can break down amygdalin into benzaldehyde, ;hydrocyanic acid and two molecules of glucose, so The bitter can be reduced greatly.

DOSAGE

l        In Gentiooligsaccharide manufacturing : glucose density 60～80%, PH 4.5 temperature 50～60℃, the dosage is 90U per gram glucose, add 1mmol/L K+, transformation period is 40～60h , the maximum accumulative total amount of Gentian oligosaccharides is 50g/L

l        Using in fermentation of the Trichoderma reesei strain to manufacture Cellulase: At the induction stage, add the above-mentioned liquid glucose according to the technology requirement. The specific dosage need to be decided according to technology characters and experiment.

Packaging

25kgs/drum; 1,125kgs/drum

Storage

Should be stored in a cool place, avoiding high temperature and insolation.

Shelf life

25℃, 6 months, enzymatic activity remains≥90%. Increase dosage after shelf life.

Safety

Enzyme preparations belong to protein, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or mucous membrane of nose, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.

Introduction
β-glucosidase (also named Cellobiase) is made from Aspergillus niger through cultivation and extraction technique. . It belongs to hydrolytic enzyme with the name of β-D-glucoside hydrolase or gentiobiase. It also contains strong glycoside activity.
Specifications/ PHYSICAL AND CHEMICAL PROPERTIES
Appearance Colour Odour pH	: Liquid with low subsidence : Brown : Normal fermentation odour : Stable 3.5-5.5, optimum 4.0-5.0
Enzymatic Activity		: ≥600 u/mL
Activity Temperature		: Stable at 40-65℃, optimum at 50-60℃
Bulk Density Calcium ion consistency		: ≤1.25g/mL : Calcium ion can protect the product
definition of unit
1 unit of β-glucosidase equals to the amount of enzyme which hydrolyzes salicin to get 1µmol reducing sugar （in glucose）at 50℃ and pH4.6 in 1min.
application
1 Production of gentiooligaccharide Enzymatic production of gentiooligaccharide mainly use specificity of cellobiase of condensation reaction, in high concentration of glucose as raw material, it can compose preferentially gentiooligaccharide of gentiobiose and gentianose etc. In this way, we can get syrup which contain high and low gentian sugar, in which there are mostly glucose apart from gentiooligaccharide 2 production of cellulase of eficient induction The gentiobiose,sophorose,sorbose,cellobiose etc which produced by glucose that transformed by glucosidase to glycosylation, is inducer of cellulase synthesis. It can increase 20% fermentation activity of cellulase, improve the brightness of liquid enzyme,improve the comprehensive quality of cellulase. 3 Improve the flavor Owing to most of precursor is in a bound form of the glycoside and no aromatic odor, BGL can work togerther with other flavor enzyme to work on β-glucoside which can break down combined fragrant material and release free fragrant material, so it can increase the aroma effect and natural flavor of fruit juice etc. 4 Extraction of polydatin Using beta glucosidase conversion technology, we can hydrolyze resveratrol with high content glucoside in the polydatin into free resveratrol, thus greatly improve the yield of resveratrol. 5 Debittering effect It can break down amygdalin into benzaldehyde, ;hydrocyanic acid and two molecules of glucose, so The bitter can be reduced greatly.