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Malachite Green (malachite green; MG) is an industrial triphenylmethane
dyes (triphenylmehtane), because of the efficacy of anti-fungal and anti-Gram-positive bacteria, and price . When breeding water containing malachite green grid cheap, so widely transported in fish farming disease control; Fish experience rapid absorption of malachite green and then metabolized into prototype malachite green (Leucomalachite green; LMG) accumulation in muscle for several months. In recent years on such dyes Toxicology found: Malachite green and its major metabolite reduced malachite green has multiple toxicity, including carcinogenicity induced gene Mutations cause chromosome breaks, the teratogenic and respiratory toxicity.
In accordance with EU standards, the residues of malachite green in fish and reduced malachite green total shall not exceed Too 2ppb (2 mcg per kg). Malachite green methods of the class of the current analysis with LC-MS and HPLC, but These methods require the more expensive the equipment and medicines, and takes a long time or special needs trained personnel side Analysis test. The company launched a malachite green enzyme immunoassay test kits, providing An analysis fast, reasonably priced and has a high sensitivity of the analytical methods.
The company launched the malachite green class enzyme immunoassay reagent kits for direct detection of the minimum 0.2ppb of prototype malachite green;, or in the use of the reducing agent, the same equivalent of malachite green Detection, and belong to triphenylmethane crystal violet (Crystal violet; CV) and reduced crystal violet (Leucocrystal Violet; LCV) cross-reactivity, and several commonly used antibiotics and sulfa drugs Classes are no cross-reactivity. This immunoassay reagents available for malachite green residues in aquatic products within The test; reagent interpretation of the cut-off set at 0.4ppb, the samples detected value is greater than 0.4ppb penalty as positive Specimen, the detected value is less than the 0.4ppb can be judged as negative specimen. We still recommend that all positive specimens must Confirmed by LC-MS analysis.
Malachite Green elisa test kit Technical specifications Sensitivity : 0.2 ppb Detection limit: shrimp, fish 0.2 ppb Recovery rate: shrimp, fish 90 ± 10% Cross-reaction rate: Reduction of malachite green (LMG) 100% Malachite Green (MG) ?? 30% Crystal violet (CV) 10% Reduction Crystal Violet (LCV) 200%