| ≥1 piece |
Pullulanase is produced from excellent strain of Bacillus licheniformis through submerged fermentation and extraction techniques. It can be widely used for industries of starch sugar.Pullulanase shows starch debranching activity and specifically catalyzes the hydrolysis of pullulan, a polysaccharide composed of maltotriose units linked through α-1,6 glycosidic bonds. The enzyme cleaves the α-1,6 glycosidic bond at the branching point and cuts the entire branch from the backbone, giving linear starch as the product. Unlike other debranching enzymes, Pullulanase can cleave most side chains including short ones. Whereas the debranching enzyme shows no activity against side chains containing only 2-3 glucose units. Therefore, pullulanase gives higher yield of linear starch.