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Agar, often referred to as agar-agar is used as a solid substrate for inclusion in culture media, gel preparation, and microbiological work. With the addition of nutritional or selection supplements, it is often used as a growth medium for bacteria and fungi. Agar is an excellent matrix for work with proteins and nucleic acids, being ideally suited for the separation of large molecules. Widely used for immunodiffusion and chromatography applications. Porous, agarose gel in varying concentrations is used as a medium for assessing the motility and mobility of microorganisms; and may also be used in the evalution of chemotaxis and chemokinesis with regard to a cell population’s movement toward a chemoattractant.